Withania somnifera chemotypes NMITLI 101R, NMITLI 118R, NMITLI 128R and withaferin A protect Mastomys coucha from Brugia malayi infection.
Identifieur interne : 003D37 ( Main/Exploration ); précédent : 003D36; suivant : 003D38Withania somnifera chemotypes NMITLI 101R, NMITLI 118R, NMITLI 128R and withaferin A protect Mastomys coucha from Brugia malayi infection.
Auteurs : S. Kushwaha [Inde] ; V K Soni ; P K Singh ; N. Bano ; A. Kumar ; R S Sangwan ; S. Misra-BhattacharyaSource :
- Parasite immunology [ 1365-3024 ] ; 2012.
Descripteurs français
- KwdFr :
- Animaux, Brugia malayi (), Brugia malayi (croissance et développement), Brugia malayi (pathogénicité), Cytokines (biosynthèse), Extraits de plantes (administration et posologie), Extraits de plantes (pharmacologie), Femelle, Filariose lymphatique (), Filariose lymphatique (immunologie), Filariose lymphatique (parasitologie), Larve (), Larve (croissance et développement), Larve (pathogénicité), Lymphocytes auxiliaires Th1 (immunologie), Lymphocytes auxiliaires Th2 (immunologie), Maladies des rongeurs (), Maladies des rongeurs (immunologie), Maladies des rongeurs (parasitologie), Murinae (parasitologie), Mâle, Plantes médicinales (), Résultat thérapeutique, Withania (), Withanolides (administration et posologie), Withanolides (pharmacologie).
- MESH :
- administration et posologie : Extraits de plantes, Withanolides.
- biosynthèse : Cytokines.
- croissance et développement : Brugia malayi, Larve.
- immunologie : Filariose lymphatique, Lymphocytes auxiliaires Th1, Lymphocytes auxiliaires Th2, Maladies des rongeurs.
- parasitologie : Filariose lymphatique, Maladies des rongeurs, Murinae.
- pathogénicité : Brugia malayi, Larve.
- pharmacologie : Extraits de plantes, Withanolides.
- Animaux, Brugia malayi, Femelle, Filariose lymphatique, Larve, Maladies des rongeurs, Mâle, Plantes médicinales, Résultat thérapeutique, Withania.
English descriptors
- KwdEn :
- Animals, Brugia malayi (drug effects), Brugia malayi (growth & development), Brugia malayi (pathogenicity), Cytokines (biosynthesis), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (parasitology), Elephantiasis, Filarial (prevention & control), Female, Larva (drug effects), Larva (growth & development), Larva (pathogenicity), Male, Murinae (parasitology), Plant Extracts (administration & dosage), Plant Extracts (pharmacology), Plants, Medicinal (chemistry), Plants, Medicinal (classification), Rodent Diseases (immunology), Rodent Diseases (parasitology), Rodent Diseases (prevention & control), Th1 Cells (immunology), Th2 Cells (immunology), Treatment Outcome, Withania (chemistry), Withania (classification), Withanolides (administration & dosage), Withanolides (pharmacology).
- MESH :
- chemical , administration & dosage : Plant Extracts, Withanolides.
- chemical , biosynthesis : Cytokines.
- chemistry : Plants, Medicinal, Withania.
- classification : Plants, Medicinal, Withania.
- drug effects : Brugia malayi, Larva.
- growth & development : Brugia malayi, Larva.
- immunology : Elephantiasis, Filarial, Rodent Diseases, Th1 Cells, Th2 Cells.
- parasitology : Elephantiasis, Filarial, Murinae, Rodent Diseases.
- pathogenicity : Brugia malayi, Larva.
- chemical , pharmacology : Plant Extracts, Withanolides.
- prevention & control : Elephantiasis, Filarial, Rodent Diseases.
- Animals, Female, Male, Treatment Outcome.
Abstract
Withania somnifera is an ayurvedic Indian medicinal plant whose immunomodulatory activities have been widely used as a home remedy for several ailments. We recently observed immunostimulatory properties in the root extracts of chemotypes NMITLI-101, NMITLI-118, NMITLI-128 and pure withanolide, withaferin A. In the present study, we evaluated the potential immunoprophylactic efficacies of these extracts against an infective pathogen. Our results show that administration of aqueous ethanol extracts (10 mg/kg) and withaferin A (0·3 mg/kg), 7 days before and after challenge with human filarial parasite Brugia malayi, offers differential protection in Mastomys coucha with chemotype 101R offering best protection (53·57%) as compared to other chemotypes. Our findings also demonstrate that establishment of B. malayi larvae was adversely affected by pretreatment with withaferin A as evidenced by 63·6% reduction in adult worm establishment. Moreover, a large percentage of the established female worms (66·2%) also showed defective embryogenesis. While the filaria-specific immunological response induced by withaferin A and NMITLI-101 showed a mixed Th1/Th2 phenotype, 118R stimulated production of IFN-γ and 128R increased levels of IL-4. Taken together, our findings reveal potential immunoprophylactic properties of W. somnifera, and further studies are needed to ascertain the benefits of this plant against other pathogens as well.
DOI: 10.1111/j.1365-3024.2012.01352.x
PubMed: 22394222
Affiliations:
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Kushwaha</name><affiliation wicri:level="1"><nlm:affiliation>Division of Parasitology, Central Drug Research Institute, A Unit of Council of Scientific and Industrial Research, Lucknow, Uttar Pradesh, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Division of Parasitology, Central Drug Research Institute, A Unit of Council of Scientific and Industrial Research, Lucknow, Uttar Pradesh</wicri:regionArea><wicri:noRegion>Uttar Pradesh</wicri:noRegion></affiliation></author><author><name sortKey="Soni, V K" sort="Soni, V K" uniqKey="Soni V" first="V K" last="Soni">V K Soni</name></author><author><name sortKey="Singh, P K" sort="Singh, P K" uniqKey="Singh P" first="P K" last="Singh">P K Singh</name></author><author><name sortKey="Bano, N" sort="Bano, N" uniqKey="Bano N" first="N" last="Bano">N. Bano</name></author><author><name sortKey="Kumar, A" sort="Kumar, A" uniqKey="Kumar A" first="A" last="Kumar">A. Kumar</name></author><author><name sortKey="Sangwan, R S" sort="Sangwan, R S" uniqKey="Sangwan R" first="R S" last="Sangwan">R S Sangwan</name></author><author><name sortKey="Misra Bhattacharya, S" sort="Misra Bhattacharya, S" uniqKey="Misra Bhattacharya S" first="S" last="Misra-Bhattacharya">S. 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Kumar</name></author><author><name sortKey="Sangwan, R S" sort="Sangwan, R S" uniqKey="Sangwan R" first="R S" last="Sangwan">R S Sangwan</name></author><author><name sortKey="Misra Bhattacharya, S" sort="Misra Bhattacharya, S" uniqKey="Misra Bhattacharya S" first="S" last="Misra-Bhattacharya">S. Misra-Bhattacharya</name></author></analytic><series><title level="j">Parasite immunology</title><idno type="eISSN">1365-3024</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Brugia malayi (drug effects)</term><term>Brugia malayi (growth & development)</term><term>Brugia malayi (pathogenicity)</term><term>Cytokines (biosynthesis)</term><term>Elephantiasis, Filarial (immunology)</term><term>Elephantiasis, Filarial (parasitology)</term><term>Elephantiasis, Filarial (prevention & control)</term><term>Female</term><term>Larva (drug effects)</term><term>Larva (growth & development)</term><term>Larva (pathogenicity)</term><term>Male</term><term>Murinae (parasitology)</term><term>Plant Extracts (administration & dosage)</term><term>Plant Extracts (pharmacology)</term><term>Plants, Medicinal (chemistry)</term><term>Plants, Medicinal (classification)</term><term>Rodent Diseases (immunology)</term><term>Rodent Diseases (parasitology)</term><term>Rodent Diseases (prevention & control)</term><term>Th1 Cells (immunology)</term><term>Th2 Cells (immunology)</term><term>Treatment Outcome</term><term>Withania (chemistry)</term><term>Withania (classification)</term><term>Withanolides (administration & dosage)</term><term>Withanolides (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Brugia malayi ()</term><term>Brugia malayi (croissance et développement)</term><term>Brugia malayi (pathogénicité)</term><term>Cytokines (biosynthèse)</term><term>Extraits de plantes (administration et posologie)</term><term>Extraits de plantes (pharmacologie)</term><term>Femelle</term><term>Filariose lymphatique ()</term><term>Filariose lymphatique (immunologie)</term><term>Filariose lymphatique (parasitologie)</term><term>Larve ()</term><term>Larve (croissance et développement)</term><term>Larve 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xml:lang="fr"><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plants, Medicinal</term><term>Withania</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Plants, Medicinal</term><term>Withania</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Brugia malayi</term><term>Larve</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Brugia malayi</term><term>Larva</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Brugia malayi</term><term>Larva</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Filariose lymphatique</term><term>Lymphocytes auxiliaires Th1</term><term>Lymphocytes auxiliaires Th2</term><term>Maladies des rongeurs</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, 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xml:lang="en"><term>Elephantiasis, Filarial</term><term>Rodent Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Male</term><term>Treatment Outcome</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Brugia malayi</term><term>Femelle</term><term>Filariose lymphatique</term><term>Larve</term><term>Maladies des rongeurs</term><term>Mâle</term><term>Plantes médicinales</term><term>Résultat thérapeutique</term><term>Withania</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Withania somnifera is an ayurvedic Indian medicinal plant whose immunomodulatory activities have been widely used as a home remedy for several ailments. We recently observed immunostimulatory properties in the root extracts of chemotypes NMITLI-101, NMITLI-118, NMITLI-128 and pure withanolide, withaferin A. In the present study, we evaluated the potential immunoprophylactic efficacies of these extracts against an infective pathogen. Our results show that administration of aqueous ethanol extracts (10 mg/kg) and withaferin A (0·3 mg/kg), 7 days before and after challenge with human filarial parasite Brugia malayi, offers differential protection in Mastomys coucha with chemotype 101R offering best protection (53·57%) as compared to other chemotypes. Our findings also demonstrate that establishment of B. malayi larvae was adversely affected by pretreatment with withaferin A as evidenced by 63·6% reduction in adult worm establishment. Moreover, a large percentage of the established female worms (66·2%) also showed defective embryogenesis. While the filaria-specific immunological response induced by withaferin A and NMITLI-101 showed a mixed Th1/Th2 phenotype, 118R stimulated production of IFN-γ and 128R increased levels of IL-4. Taken together, our findings reveal potential immunoprophylactic properties of W. somnifera, and further studies are needed to ascertain the benefits of this plant against other pathogens as well.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Bano, N" sort="Bano, N" uniqKey="Bano N" first="N" last="Bano">N. Bano</name><name sortKey="Kumar, A" sort="Kumar, A" uniqKey="Kumar A" first="A" last="Kumar">A. Kumar</name><name sortKey="Misra Bhattacharya, S" sort="Misra Bhattacharya, S" uniqKey="Misra Bhattacharya S" first="S" last="Misra-Bhattacharya">S. Misra-Bhattacharya</name><name sortKey="Sangwan, R S" sort="Sangwan, R S" uniqKey="Sangwan R" first="R S" last="Sangwan">R S Sangwan</name><name sortKey="Singh, P K" sort="Singh, P K" uniqKey="Singh P" first="P K" last="Singh">P K Singh</name><name sortKey="Soni, V K" sort="Soni, V K" uniqKey="Soni V" first="V K" last="Soni">V K Soni</name></noCountry><country name="Inde"><noRegion><name sortKey="Kushwaha, S" sort="Kushwaha, S" uniqKey="Kushwaha S" first="S" last="Kushwaha">S. Kushwaha</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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